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Shichi and Hackett have isolated 3 b-type cytochromes from etiolated mung bean seedlings using conventional methods of ammonium sulfate fractionation and column chromatography (3, 5). Because of the turbidity of some of their early extracts. they were unable to measure the difference spectrum of their samples and therefore could not calculate the overall yield and purity of their cytochrome preparations. We have repeated some of their work using the scattered transmission accessory (Cary model 1462) for the Cary Model 14 recording spectrophotometer to measure the difference spectra of strongly scattering suspensions. From the heights of the a bands we have calculated the b-type cytochrome content at each step during the purification procedure.

Calculation of Purity and Yields in the Purification of b-Type Cytochromes from Etiolated Seedlings of Phaseolus aureus