Metabolism of Sinapine in Mustard Plants. II. Purification & Some Properties of Sinapine Esterase | Zendy

Alexander Tzagoloff | Zendy

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Metabolism of Sinapine in Mustard Plants. II. Purification & Some Properties of Sinapine Esterase

Author(s) -

Alexander Tzagoloff

Publication year - 1963

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.38.2.207

Subject(s) - metabolism , chemistry , botany , biochemistry , biology

Experimental evidence for the presence in mustard seedlings of an esterase that catalyzes the hydrolysis of sinapine into sinapic acid and choline has been presented previously (12). It was felt that further studies on this enzyme might be of some general biochemical interest. A preparation of sinapine esterase purified 20fold has been obtained from white mustard seedlings (Brassica hirta Moench). The specificity, pH optimum, and other properties of the enzyme have been examined and will be discussed in this paper. A spectrophotometric method for following the hydrolysis of sinapine based on the absorption of sinapic acid at 290 myt has been devised and is described.

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