Nicotine Production and Growth of Tobacco Scions on Tomato Rootstocks.

Excised roots of Turkish tobacco were grown in sterile culture, and a detailed study was made of the interrelations of their growth and nicotine prodtuction. During the exponential phase of growth the roots produced nicotine at a constant rate of 0.191 )gm per mm length or 29.05 lgm l)er mg dry weight. Wlhen the growing tips and matture regions of the root were cultured separately, nicotine increases resulted only in the case of the tips. By the use of indoleacetic acid in proper concentration, nicotine synthesis was ohservedl only in those roots where elongation occurred.