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Enzymatic Synthesis of Glutamine in Higher Plants
Author(s) -
George C. Webster
Publication year - 1953
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.28.4.724
Subject(s) - acetone , residue (chemistry) , chemistry , potassium , glutamine , sodium , chromatography , enzyme , suspension (topology) , sodium glutamate , potassium phosphate , phosphate , nuclear chemistry , raw material , organic chemistry , biochemistry , mathematics , homotopy , pure mathematics , amino acid
volumes of chilled acetone in a Waring Blendor at Io C. The suspension was filtered with suction at Io C, and the residue sucked dry in a stream of cold air. The operation was repeated, and the coarse powder was sifted through a screen. The resulting powder was stored in a desiccator at Io C. Active extracts of acetone powders were prepared essentially in the manner described by Speck (10). Unless otherwise indicated, the complete reaction system contained: 0.05 M potassium phosphate buffer (pH 7.0), 0.05 M sodium L-glutamate,

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