Iron–Sulfur Cluster Protein NITROGEN FIXATION S-LIKE1 and Its Interactor FRATAXIN Function in Plant Immunity | Zendy

Jose Pedro Fonseca | Zendy; HeeKyung Lee | Zendy; Clarissa Boschiero | Zendy; Marcus Griffiths | Zendy; Seonghee Lee | Zendy; Patrick X. Zhao | Zendy; Larry M. York | Zendy; Kirankumar S. Mysore | Zendy

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Iron–Sulfur Cluster Protein NITROGEN FIXATION S-LIKE1 and Its Interactor FRATAXIN Function in Plant Immunity

Author(s) -

Jose Pedro Fonseca,

HeeKyung Lee,

Clarissa Boschiero,

Marcus Griffiths,

Seonghee Lee,

Patrick X. Zhao,

Larry M. York,

Kirankumar S. Mysore

Publication year - 2020

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.20.00950

Subject(s) - pseudomonas syringae , arabidopsis , biology , nicotiana benthamiana , plant disease resistance , interactor , arabidopsis thaliana , gene , innate immune system , genetics , mutant , plant defense against herbivory , frataxin , microbiology and biotechnology , immune system , iron binding proteins

Iron-sulfur (Fe-S) clusters are inorganic cofactors that are present in all kingdoms of life as part of a large number of proteins involved in several cellular processes, including DNA replication and metabolism. In this work, we demonstrate an additional role for two Fe-S cluster genes in biotic stress responses in plants. Eleven Fe-S cluster genes, including the NITROGEN FIXATION S - LIKE1 ( NFS1 ) and its interactor FRATAXIN ( FH ), when silenced in Nicotiana benthamiana , compromised nonhost resistance to Pseudomonas syringae pv. tomato T1. NbNFS1 expression was induced by pathogens and salicylic acid. Arabidopsis ( Arabidopsis thaliana ) atnfs and atfh mutants, with reduced AtNFS1 or AtFH gene expression, respectively, showed increased susceptibility to both host and nonhost pathogen infection. Arabidopsis AtNFS1 and AtFH overexpressor lines displayed decreased susceptibility to infection by host pathogen P syringae pv. tomato DC3000. The AtNFS1 overexpression line exhibited constitutive upregulation of several defense-related genes and enrichment of gene ontology terms related to immunity and salicylic acid responses. Our results demonstrate that NFS1 and its interactor FH are involved not only in nonhost resistance but also in basal resistance, suggesting a new role of the Fe-S cluster pathway in plant immunity.

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