Phosphorylation-Mediated Dynamics of Nitrate Transceptor NRT1.1 Regulate Auxin Flux and Nitrate Signaling in Lateral Root Growth | Zendy

Xi Zhang | Zendy; Yaning Cui | Zendy; Meng Yu | Zendy; Bodan Su | Zendy; Wei Gong | Zendy; František Baluška | Zendy; George Komis | Zendy; Jozef Šamaj | Zendy; Xiaoyi Shan | Zendy; Jinxing Lin | Zendy

Open Access

Phosphorylation-Mediated Dynamics of Nitrate Transceptor NRT1.1 Regulate Auxin Flux and Nitrate Signaling in Lateral Root Growth

Author(s) -

Xi Zhang,

Yaning Cui,

Meng Yu,

Bodan Su,

Wei Gong,

František Baluška,

George Komis,

Jozef Šamaj,

Xiaoyi Shan,

Jinxing Lin

Publication year - 2019

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.19.00346

Subject(s) - arabidopsis , auxin , phosphorylation , chemistry , lateral root , microbiology and biotechnology , arabidopsis thaliana , endocytosis , nitrate , signal transduction , biochemistry , mutant , biology , gene , receptor , organic chemistry

The dual-affinity nitrate transceptor NITRATE TRANSPORTER1.1 (NRT1.1) has two modes of transport and signaling, governed by Thr-101 (T101) phosphorylation. NRT1.1 regulates lateral root (LR) development by modulating nitrate-dependent basipetal auxin export and nitrate-mediated signal transduction. Here, using the Arabidopsis ( Arabidopsis thaliana ) NRT1.1 T101D phosphomimetic and NRT1.1 T101A nonphosphorylatable mutants, we found that the phosphorylation state of NRT1.1 plays a key role in NRT1.1 function during LR development. Single-particle tracking revealed that phosphorylation affected NRT1.1 spatiotemporal dynamics. The phosphomimetic NRT1.1 T101D form showed fast lateral mobility and membrane partitioning that facilitated auxin flux under low-nitrate conditions. By contrast, nonphosphorylatable NRT1.1 T101A showed low lateral mobility and oligomerized at the plasma membrane (PM), where it induced endocytosis via the clathrin-mediated endocytosis and microdomain-mediated endocytosis pathways under high-nitrate conditions. These behaviors promoted LR development by suppressing NRT1.1-controlled auxin transport on the PM and stimulating Ca 2+ -ARABIDOPSIS NITRATE REGULATED1 signaling from the endosome.

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