A GPI Signal Peptide-Anchored Split-Ubiquitin (GPS) System for Detecting Soluble Bait Protein Interactions at the Membrane
Author(s) -
Ben Zhang,
Rucha Karnik,
Naomi Donald,
Michael R. Blatt
Publication year - 2018
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.18.00577
Subject(s) - ubiquitin , global positioning system , signal peptide , peptide , microbiology and biotechnology , signal (programming language) , membrane , gps signals , membrane protein , chemistry , biology , biochemistry , computer science , assisted gps , peptide sequence , gene , telecommunications , programming language
Protein-protein interactions play critical roles in a wide range of cellular functions. The need to analyze these interactions has spawned a variety of biophysical and biochemical techniques, including coimmunoprecipitation, yeast two-hybrid, bimolecular fluorescence complementation, Forster
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