Soft X-Ray Imaging of Cellular Carbon and Nitrogen Distributions in Heterocystous Cyanobacteria | Zendy

Takahiro Teramoto | Zendy; Chihiro Azai | Zendy; Kazuki Terauchi | Zendy; Masashi Yoshimura | Zendy; Toshiaki Ohta | Zendy

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Soft X-Ray Imaging of Cellular Carbon and Nitrogen Distributions in Heterocystous Cyanobacteria

Author(s) -

Takahiro Teramoto,

Chihiro Azai,

Kazuki Terauchi,

Masashi Yoshimura,

Toshiaki Ohta

Publication year - 2018

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.17.01767

Subject(s) - heterocyst , cyanobacteria , anabaena , nitrogen , nitrogen fixation , photosynthesis , carbon fibers , botany , nitrogenase , biophysics , biology , chemistry , materials science , bacteria , organic chemistry , composite number , composite material , genetics

Soft x-ray microscopy (SXM) is a minimally invasive technique for single-cell high-resolution imaging as well as the visualization of intracellular distributions of light elements such as carbon, nitrogen, and oxygen. We used SXM to observe photosynthesis and nitrogen fixation in the filamentous cyanobacterium Anabaena sp. PCC 7120, which can form heterocysts during nitrogen starvation. Statistical and spectroscopic analyses from SXM images around the K-absorption edge of nitrogen revealed a significant difference in the carbon-to-nitrogen (C/N) ratio between vegetative cells and heterocysts. Application of this analysis to soft x-ray images of Anabaena sp. PCC 7120 revealed inhomogenous C/N ratios in the cells. Furthermore, soft x-ray tomography of Anabaena sp. PCC 7120 revealed differing cellular C/N ratios, indicating different carbon and nitrogen distributions between vegetative cells and heterocysts in three dimensions.

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