A Tightly Regulated Genetic Selection System with Signaling-Active Alleles of Phytochrome B | Zendy

Wei Hu | Zendy; J. Clark Lagarias | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A Tightly Regulated Genetic Selection System with Signaling-Active Alleles of Phytochrome B

Author(s) -

Wei Hu,

J. Clark Lagarias

Publication year - 2016

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.16.01345

Subject(s) - biology , selectable marker , arabidopsis , gene , arabidopsis thaliana , allele , genetics , transgene , phenotype , selection (genetic algorithm) , phytochrome , genetically modified crops , mutant , botany , red light , artificial intelligence , computer science

Selectable markers derived from plant genes circumvent the potential risk of antibiotic/herbicide-resistance gene transfer into neighboring plant species, endophytic bacteria, and mycorrhizal fungi. Toward this goal, we have engineered and validated signaling-active alleles of phytochrome B (eYHB) as plant-derived selection marker genes in the model plant Arabidopsis (Arabidopsis thaliana). By probing the relationship of construct size and induction conditions to optimal phenotypic selection, we show that eYHB-based alleles are robust substitutes for antibiotic/herbicide-dependent marker genes as well as surprisingly sensitive reporters of off-target transgene expression.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research