A Medicago truncatula Homoglutathione Synthetase Is Derived from Glutathione Synthetase by Gene Duplication | Zendy

Pierre Frendo | Zendy; Marı́a Jesús Hernández Jiménez | Zendy; Chantal Mathieu | Zendy; Laurent Duret | Zendy; Daniela Gallesi | Zendy; Ghislaine Van de Sype | Zendy; Didier Hérouart | Zendy; Alain Puppo | Zendy

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A Medicago truncatula Homoglutathione Synthetase Is Derived from Glutathione Synthetase by Gene Duplication

Author(s) -

Pierre Frendo,

Marı́a Jesús Hernández Jiménez,

Chantal Mathieu,

Laurent Duret,

Daniela Gallesi,

Ghislaine Van de Sype,

Didier Hérouart,

Alain Puppo

Publication year - 2001

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.126.4.1706

Subject(s) - medicago truncatula , gene duplication , glutathione synthetase , gene , glutathione , biology , genetics , enzyme , biochemistry , symbiosis , bacteria

Glutathione (GSH) and homo-GSH (hGSH) are the major low-molecular weight thiols synthesized in Medicago truncatula. Two M. truncatula cDNAs (gshs1 and gshs2) corresponding to a putative GSH synthetase (GSHS) and a putative hGSH synthetase (hGSHS) were characterized. Heterologous expression of gshs1 and gshs2 cDNAs in an Escherichia coli strain deficient in GSHS activity showed that GSHS1 and GSHS2 are a GSHS and an hGSHS, respectively. Leucine-534 and proline-535 present in hGSHS were substituted by alanines that are conserved in plant GSHS. These substitutions resulted in a strongly stimulated GSH accumulation in the transformed E. coli strain showing that these residues play a crucial role in the differential recognition of beta-alanine and glycine by hGSHS. Phylogenetic analysis of GSHS2 and GSHS1 with other eukaryotic GSHS sequences indicated that gshs2 and gshs1 are the result of a gene duplication that occurred after the divergence between Fabales, Solanales, and Brassicales. Analysis of the structure of gshs1 and gshs2 genes shows they are both present in a cluster and in the same orientation in the M. truncatula genome, suggesting that the duplication of gshs1 and gshs2 occurred via a tandem duplication.

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