Cysteine Synthase Overexpression in Tobacco Confers Tolerance to Sulfur-Containing Environmental Pollutants | Zendy

Masaaki Noji | Zendy; Maiko Saito | Zendy; Michimi Nakamura | Zendy; Mitsuko Aono | Zendy; Hikaru Saji | Zendy; Kazuki Saito | Zendy

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Cysteine Synthase Overexpression in Tobacco Confers Tolerance to Sulfur-Containing Environmental Pollutants

Author(s) -

Masaaki Noji,

Maiko Saito,

Michimi Nakamura,

Mitsuko Aono,

Hikaru Saji,

Kazuki Saito

Publication year - 2001

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.126.3.973

Subject(s) - nicotiana tabacum , glutathione , cysteine , paraquat , sulfur , sulfite , biochemistry , chloroplast , fumigation , genetically modified crops , cytosol , biosynthesis , chemistry , biology , atp synthase , transgene , enzyme , gene , horticulture , organic chemistry

Cysteine (Cys) synthase [O-acetyl-L-Ser(thiol)-lyase, EC 4.2.99.8; CSase] is responsible for the final step in biosynthesis of Cys. Transgenic tobacco (Nicotiana tabacum; F(1)) plants with enhanced CSase activities in the cytosol and in the chloroplasts were generated by cross-fertilization of two transformants expressing cytosolic CSase or chloroplastic CSase. The F(1) transgenic plants were highly tolerant to toxic sulfur dioxide and sulfite. Upon fumigation with 0.1 microL L(-1) sulfur dioxide, the Cys and glutathione contents in leaves of F(1) plants were increased significantly, but not in leaves of non-transformed control plants. Furthermore, the leaves of F(1) plants exhibited the increased resistance to paraquat, a herbicide generating active oxygen species.

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