Glucose and Disaccharide-Sensing Mechanisms Modulate the Expression of α-amylase in Barley Embryos | Zendy

Elena Loreti | Zendy; Amedeo Alpi | Zendy; Pierdomenico Perata | Zendy

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Glucose and Disaccharide-Sensing Mechanisms Modulate the Expression of α-amylase in Barley Embryos

Author(s) -

Elena Loreti,

Amedeo Alpi,

Pierdomenico Perata

Publication year - 2000

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.123.3.939

Subject(s) - disaccharide , sucrose , biochemistry , chemistry , fructose , galactose , moiety , carbohydrate , trehalase , enzyme , stereochemistry

The aim of this study was to investigate the sugar-sensing processes modulating the expression of alpha-amylase in barley (Hordeum vulgaris L. var Himalaya) embryos. The results highlight the existence of independent glucose (Glc) and disaccharides sensing. Glc treatment destabilizes the alpha-amylase mRNA. Non-metabolizable disaccharides repress alpha-amylase induction, but have no effects on transcript stability. Structure-function analysis indicates that a fructose (Fru) moiety is needed for disaccharide sensing. Lactulose (beta-galactose [Gal][1-->4]Fru), palatinose (Glc[1-->6]Fru), and turanose (Glc[1-->3]Fru) are not metabolized but repress alpha-amylase. Disrupting the fructosyl moiety of lactulose and palatinose, or replacing the Fru moiety of beta-Gal[1-->4]Fru with Glc or Gal results in molecules unable to repress alpha-amylase. Comparison of the molecular requirements for sucrose transport with those for disaccharide sensing suggests that these sugars are perceived possibly at the plasma membrane level independently from sucrose transport.

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