Nucleoside Diphosphate Kinase Required for Coleoptile Elongation in Rice

Although several nucleoside diphosphate (NDP) kinase genes have been cloned in plants, little is known about the functional significance of this enzyme during plant growth and development. We introduced a chimeric gene encoding an antisense RNA of NDP kinase under the control of the Arabidopsis heat shock protein HSP81-1 promoter into rice (Oryza sativaL.) plants using the Agrobacterium tumefacienstransformation system. The expression of antisense RNA down-regulated the accumulation of mRNA, resulting in reduced enzyme activity even under the standard growth temperature (25°C) in transgenic plants. Following heat shock treatment (37°C), NDP kinase activities in some transgenic rice plants were more reduced than those grown under 25°C. The comparison of the coleoptile growth under submersion showed that cell elongation process was inhibited in antisense NDP kinase transgenic plants, suggesting that an altered guanine nucleotide level may be responsible for the processes.