Open AccessIron Deficiency Decreases the Fe(III)-Chelate Reducing Activity of Leaf Protoplasts
Author(s) -
Elena B. González-Vallejo,
Fermı́n Morales,
L. Cistué,
Anunciación Abadı́a,
Javier Abadı́a
Publication year - 2000
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.122.2.337
Subject(s) - protoplast , apoplast , iron deficiency , sugar beet , chelation , chemistry , biology , botany , biochemistry , biophysics , cell wall , horticulture , inorganic chemistry , medicine , anemia
The ferric-chelate reductase (FC-R) activity of mesophyll protoplasts isolated from Fe-sufficient (control) and Fe-deficient sugar beet (Beta vulgaris L.) leaves has been characterized. Measurements were made in an ionic environment similar to that in the apoplastic space of the sugar beet mesophyll cells. The FC-R activity of Fe-sufficient and Fe-deficient protoplasts was dependent on light. Fe deficiency decreased markedly the FC-R activity per protoplast surface unit. The optimal pH for the activity of the FC-R in mesophyll protoplasts was in the range 5.5 to 6.0, typical of the apoplastic space. Beyond pH 6.0, the activity of the FC-R in mesophyll protoplasts decreased markedly in both Fe-sufficient and Fe-deficient protoplasts. These data suggest that both the intrinsic decrease in FC-R activity per protoplast surface and a possible shift in the pH of the apoplastic space could lead to the accumulation of physiologically inactive Fe pools in chlorotic leaves.
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