Mannose Induces an Endonuclease Responsible for DNA Laddering in Plant Cells | Zendy

Joshua C. Stein | Zendy; Geneviève Hansen | Zendy

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Mannose Induces an Endonuclease Responsible for DNA Laddering in Plant Cells

Author(s) -

Joshua C. Stein,

Geneviève Hansen

Publication year - 1999

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.121.1.71

Subject(s) - dna laddering , dna fragmentation , apoptosis , biochemistry , biology , endonuclease , fragmentation (computing) , dna , microbiology and biotechnology , mannose , programmed cell death , ecology

The effect of D-mannose (Man) on plant cells was studied in two different systems: Arabidopsis roots and maize (Zea mays) suspension-cultured cells. In both systems, exposure to D-Man was associated with a subset of features characteristic of apoptosis, as assessed by oligonucleosomal fragmentation and microscopy analysis. Furthermore, D-Man induced the release of cytochrome c from mitochondria. The specificity of D-Man was evaluated by comparing the effects of diastereomers such as L-Man, D-glucose, and D-galactose. Of these treatments, only D-Man caused a reduction in final fresh weight with concomitant oligonucleosomal fragmentation. Man-induced DNA laddering coincided with the activation of a DNase in maize cytosolic extracts and with the appearance of single 35-kD band detected using an in-gel DNase assay. The DNase activity was further confirmed by using covalently closed circular plasmid DNA as a substrate. It appears that D-Man, a safe and readily accessible compound, offers remarkable features for the study of apoptosis in plant cells.

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