Arabidopsis ent-Kaurene Oxidase Catalyzes Three Steps of Gibberellin Biosynthesis | Zendy

Chris A. Helliwell | Zendy; Andrew T. Poole | Zendy; W. James Peacock | Zendy; Elizabeth S. Dennis | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Arabidopsis ent-Kaurene Oxidase Catalyzes Three Steps of Gibberellin Biosynthesis

Author(s) -

Chris A. Helliwell,

Andrew T. Poole,

W. James Peacock,

Elizabeth S. Dennis

Publication year - 1999

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.119.2.507

Subject(s) - arabidopsis , gibberellin , yeast , biosynthesis , biochemistry , oxidase test , saccharomyces cerevisiae , enzyme , complementary dna , chemistry , biology , gene , botany , mutant

The Arabidopsis GA3 cDNA was expressed in yeast (Saccharomyces cerevisiae) and the ability of the transformed yeast cells to metabolize ent-kaurene was tested. We show by full-scan gas chromatography-mass spectrometry that the transformed cells produce ent-kaurenoic acid, and demonstrate that the single enzyme GA3 (ent-kaurene oxidase) catalyzes the three steps of gibberellin biosynthesis from ent-kaurene to ent-kaurenoic acid.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research