Occurrence of Cello-Oligosaccharides in the Apoplast of Auxin-Treated Pea Stems | Zendy

Rumi Tominaga | Zendy; Masahiro Samejima | Zendy; Fukumi Sakai | Zendy; Takahisa Hayashi | Zendy

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Occurrence of Cello-Oligosaccharides in the Apoplast of Auxin-Treated Pea Stems

Author(s) -

Rumi Tominaga,

Masahiro Samejima,

Fukumi Sakai,

Takahisa Hayashi

Publication year - 1999

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.119.1.249

Subject(s) - xyloglucan , apoplast , pisum , cello , chemistry , biochemistry , oligosaccharide , auxin , fucose , epicotyl , botany , chromatography , polysaccharide , biology , cell wall , hypocotyl , glycoprotein , art , piano , gene , art history

Treatment of pea (Pisum sativum L.) hypocotyl segments with indole-3-butyric acid, which promotes segment elongation, increased the solubilization of both xyloglucan and cello-oligosaccharides in the apoplast of auxin-treated pea stems. The cello-oligosaccharides were isolated from the apoplastic solution with a charcoal/Celite column and were identified as cellobiose, cellotriose, and cellotetraose after subsequent thin-layer chromatography and paper electrophoresis. Cello-oligosaccharides in the apoplastic fraction were monitored using cellobiose dehydrogenase. Both xyloglucan and cello-oligosaccharides appeared to be formed concurrently within 30 min after treatment with the auxin, and the cello-oligosaccharides increased with stem elongation even after 2 h. The total activity of cellulase did not increase for up to 4 h.

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