Phloem-Specific Expression of the Tobacco Mosaic Virus Movement Protein Alters Carbon Metabolism and Partitioning in Transgenic Potato Plants | Zendy

Einat Almon | Zendy; Michal Horowitz | Zendy; H. L. Wang | Zendy; William J. Lucas | Zendy; E. Zamski | Zendy; Sebastian Wolf | Zendy

Open Access

Phloem-Specific Expression of the Tobacco Mosaic Virus Movement Protein Alters Carbon Metabolism and Partitioning in Transgenic Potato Plants

Author(s) -

Einat Almon,

Michal Horowitz,

H. L. Wang,

William J. Lucas,

E. Zamski,

Sebastian Wolf

Publication year - 1997

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.115.4.1599

Subject(s) - tobacco mosaic virus , phloem , nicotiana tabacum , biology , movement protein , sucrose , solanaceae , cauliflower mosaic virus , genetically modified crops , starch , botany , transgene , metabolism , tobacco etch virus , nicotiana , solanum tuberosum , potato virus x , plasmodesma , biochemistry , plant virus , cell wall , gene , virus , potyvirus , coat protein , rna , virology

The tobacco mosaic virus movement protein (TMV-MP) has pleiotropic effects when expressed in transgenic tobacco (Nicotiana tabacum) plants. In addition to its ability to increase the plasmodesmal size-exclusion limit, the TMV-MP alters carbohydrate metabolism in source leaves and dry matter partitioning between the various plant organs. In the present study the TMV-MP was expressed under the control of a phloem-specific promoter (rolC), and this system was employed to further explore the potential sites at which the TMV-MP exerts its influence over carbon metabolism and transport in transgenic potato (Solanum tuberosum) plants. Immunohistochemical analyses indicated that the TMV-MP was localized mainly to phloem parenchyma and companion cells. Starch and sucrose accumulated in source leaves of these plants to significantly higher levels compared with control potato lines. In addition, the rate of sucrose efflux from excised petioles was lower compared with control plants. Furthermore, under short-day conditions, carbon partitioning was lower to the roots and higher to tubers in rolC plants compared with controls. These results are discussed in terms of the mode(s) by which the TMV-MP exerts its influence over carbon metabolism and photoassimilate translocation.

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