An Optical Clearing Technique for Plant Tissues Allowing Deep Imaging and Compatible with Fluorescence Microscopy  | Zendy

Cherish Warner | Zendy; Meredith L. Biedrzycki | Zendy; Samuel S. Jacobs | Zendy; Randall J. Wisser | Zendy; Jeffrey L. Caplan | Zendy; D. Janine Sherrier | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

An Optical Clearing Technique for Plant Tissues Allowing Deep Imaging and Compatible with Fluorescence Microscopy

Author(s) -

Cherish Warner,

Meredith L. Biedrzycki,

Samuel S. Jacobs,

Randall J. Wisser,

Jeffrey L. Caplan,

D. Janine Sherrier

Publication year - 2014

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.114.244673

Subject(s) - clearing , fluorescence , immunocytochemistry , microscopy , fluorescence microscope , fluorescence lifetime imaging microscopy , microscope , optics , materials science , biophysics , biology , physics , finance , economics , endocrinology

We report on a nondestructive clearing technique that enhances transmission of light through specimens from diverse plant species, opening unique opportunities for microscope-enabled plant research. After clearing, plant organs and thick tissue sections are amenable to deep imaging. The clearing method is compatible with immunocytochemistry techniques and can be used in concert with common fluorescent probes, including widely adopted protein tags such as GFP, which has fluorescence that is preserved during the clearing process.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research