Open AccessComplementary DNAs Encoding Eukaryotic-Type Cytidine-5[prime]-Diphosphate-Diacylglycerol Synthases of Two Plant Species
Author(s) -
Joachim Kopka,
Michael H. Ludewig,
Bernd MüllerRöber
Publication year - 1997
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.113.3.997
Subject(s) - diacylglycerol kinase , biochemistry , cardiolipin , biology , cytidine , arabidopsis thaliana , atp synthase , escherichia coli , arabidopsis , gene , enzyme , phospholipid , mutant , protein kinase c , membrane
Cytidine diphosphate (CDP)-diacylglycerol synthase (cytidine triphosphate:phosphatidate cytihyltransferase, EC 2.7.7.41) catalyzes the formation of CDP-diacylglycerol, which is the precursor of phosphatidylinositol, phosphatidylglycerol, and cardiolipin. We report the first cloning, to our knowledge, of two plant cDNAs, StCDS1 and AtCDS1, coding for CDP-diacylglycerol synthase from potato (Solanum tuberosum) and Arabidopsis thaliana, respectively. The two proteins belong to the eukaryotic type of CDP-diacylglycerol synthase and contain eight predicted transmembrane-spanning domains. We analyzed gene expression in shoot and root tissues of potato plants and demonstrated enzyme activity by expression of N-terminally truncated, recombinant StCDS1 in Escherichia coli.