Open AccessThe Role of TIR-NBS and TIR-X Proteins in Plant Basal Defense Responses
Author(s) -
Raja Sekhar Nandety,
Jeffrey L. Caplan,
Kerri L. Cavanaugh,
Bertrand Perroud,
Tadeusz Wróblewski,
Richard W. Michelmore,
Blake C. Meyers
Publication year - 2013
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.113.219162
Subject(s) - arabidopsis , nicotiana benthamiana , biology , effector , arabidopsis thaliana , chlorosis , jasmonate , gene , microbiology and biotechnology , mutant , genetics , botany
Toll/interleukin receptor (TIR) domain-containing proteins encoded in the Arabidopsis (Arabidopsis thaliana) genome include the TIR-nucleotide binding site (TN) and TIR-unknown site/domain (TX) families. We investigated the function of these proteins. Transient overexpression of five TX and TN genes in tobacco (Nicotiana benthamiana) induced chlorosis. This induced chlorosis was dependent on ENHANCED DISEASE RESISTANCE1, a dependency conserved in both tobacco and Arabidopsis. Stable overexpression transgenic lines of TX and TN genes in Arabidopsis produced a variety of phenotypes associated with basal innate immune responses; these were correlated with elevated levels of salicylic acid. The TN protein AtTN10 interacted with the chloroplastic protein phosphoglycerate dehydrogenase in a yeast (Saccharomyces cerevisiae) two-hybrid screen; other TX and TN proteins interacted with nucleotide binding-leucine-rich repeat proteins and effector proteins, suggesting that TN proteins might act in guard complexes monitoring pathogen effectors.
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