Open AccessSulfur Availability and the SAC1 Gene Control Adenosine Triphosphate Sulfurylase Gene Expression in Chlamydomonas reinhardtii
Author(s) -
Fitnat H. Yildiz,
John P. Davies,
Arthur Grossman
Publication year - 1996
Publication title -
plant physiology (bethesda)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.112.2.669
Subject(s) - chlamydomonas reinhardtii , biochemistry , biology , gene , structural gene , adenosine triphosphate , complementary dna , nucleotide , escherichia coli , microbiology and biotechnology , mutant
A Chlamydomonas reinhardtii adenosine triphosphate (ATP) sulfurylase cDNA clone (pATS1) was selected by complementing a mutation in the ATP sulfurylase gene (cysD) of Escherichia coli. E. coli cysD strains harboring pATS1 grow on medium containing sulfate as the sole sulfur source and exhibit ATP sulfurylase activity. The amino acid sequence of the C. reinhardtii ATP sulfurylase, derived from the nucleotide sequence of the complementing gene (ATS1), is 25 to 40% identical to that of ATP sulfurylases in other eukaryotic organisms and has a putative transit peptide at its amino terminus. ATP sulfurylase mRNA was present when cells were grown in sulfur-replete medium, but accumulated to higher levels when the cells were exposed to sulfur-limiting conditions. Furthermore, sulfur-stress-induced accumulation of the ATS1 transcript was reduced in a strain defective in SAC1, a gene that is critical for acclimation to sulfur-limited growth.