Functional Expression of the Extraplastidial Arabidopsis thaliana Oleate Desaturase Gene (FAD2) in Saccharomyces cerevisiae | Zendy

Patrick S. Covello | Zendy; Darwin W. Reed | Zendy

Open Access

Functional Expression of the Extraplastidial Arabidopsis thaliana Oleate Desaturase Gene (FAD2) in Saccharomyces cerevisiae

Author(s) -

Patrick S. Covello,

Darwin W. Reed

Publication year - 1996

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.111.1.223

Subject(s) - saccharomyces cerevisiae , arabidopsis thaliana , fatty acid desaturase , yeast , linoleic acid , biochemistry , gene , biology , arabidopsis , fatty acid , gene expression , chemistry , polyunsaturated fatty acid , mutant

The functional expression in yeast of the Arabidopsis thaliana FAD2 gene, encoding the extraplastidial oleate desaturase (1-acyl-2-oleoyl-sn-glycero-3-phosphocholine delta 12-desaturase) is reported. Dienoic fatty acids constituted up to 11% (w/w) of the total fatty acids in transformed Saccharomyces cerevisiae cells and were confirmed to be linoleic acid and delta 9, delta 12-hexadecadienoic acid by gas chromatography-mass spectrometry.

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