Suppression of a NAC-Like Transcription Factor Gene Improves Boron-Toxicity Tolerance in Rice | Zendy

Kumiko Ochiai | Zendy; Akifumi Shimizu | Zendy; Yutaka Okumoto | Zendy; Toru Fujiwara | Zendy; Tōru Matoh | Zendy

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Suppression of a NAC-Like Transcription Factor Gene Improves Boron-Toxicity Tolerance in Rice

Author(s) -

Kumiko Ochiai,

Akifumi Shimizu,

Yutaka Okumoto,

Toru Fujiwara,

Tōru Matoh

Publication year - 2011

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.110.171470

Subject(s) - biology , gene , transgene , oryza sativa , toxicity , sulfonylurea , genetically modified rice , genetically modified crops , genetics , microbiology and biotechnology , chemistry , organic chemistry , insulin

We identified a gene responsible for tolerance to boron (B) toxicity in rice (Oryza sativa), named BORON EXCESS TOLERANT1. Using recombinant inbred lines derived from the B-toxicity-sensitive indica-ecotype cultivar IR36 and the tolerant japonica-ecotype cultivar Nekken 1, the region responsible for tolerance to B toxicity was narrowed to 49 kb on chromosome 4. Eight genes are annotated in this region. The DNA sequence in this region was compared between the B-toxicity-sensitive japonica cultivar Wataribune and the B-toxicity-tolerant japonica cultivar Nipponbare by eco-TILLING analysis and revealed a one-base insertion mutation in the open reading frame sequence of the gene Os04g0477300. The gene encodes a NAC (NAM, ATAF, and CUC)-like transcription factor and the function of the transcript is abolished in B-toxicity-tolerant cultivars. Transgenic plants in which the expression of Os04g0477300 is abolished by RNA interference gain tolerance to B toxicity.

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