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Expression of an Arabidopsis Potassium Channel Gene in Guard Cells
Author(s) -
Robert L. Nakamura,
William L. McKendree,
Rhoda Elison Hirsch,
John C. Sedbrook,
Richard F. Gaber,
Michael R. Sussman
Publication year - 1995
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.109.2.371
Subject(s) - arabidopsis , gene , guard cell , coding region , complementary dna , biology , reporter gene , intron , arabidopsis thaliana , genomic dna , microbiology and biotechnology , genetics , promoter , gene expression , transgene , glucuronidase , mutant
The Arabidopsis thaliana KAT1 cDNA encodes a voltage-gated inward-rectifying K+ channel. A KAT1 genomic DNA clone was isolated and sequenced, and a 5' promoter and coding sequences containing eight introns were identified. Reporter gene analysis of transgenic plants containing the KAT1 promoter fused to bacterial beta-glucuronidase showed robust beta-glucuronidase activity primarily in guard cells.

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