Modulation of Transcriptome and Metabolome of Tobacco by Arabidopsis Transcription Factor, AtMYB12, Leads to Insect Resistance | Zendy

Prashant Misra | Zendy; Ashutosh Pandey | Zendy; Manish Tiwari | Zendy; K. Chandrashekar | Zendy; Om P. Sidhu | Zendy; Mehar Hasan Asif | Zendy; Debasis Chakrabarty | Zendy; Pradhyumna Kumar Singh | Zendy; Prabodh Kumar Trivedi | Zendy; Pravendra Nath | Zendy; Rakesh Tuli | Zendy

Open Access

Modulation of Transcriptome and Metabolome of Tobacco by Arabidopsis Transcription Factor, AtMYB12, Leads to Insect Resistance

Author(s) -

Prashant Misra,

Ashutosh Pandey,

Manish Tiwari,

K. Chandrashekar,

Om P. Sidhu,

Mehar Hasan Asif,

Debasis Chakrabarty,

Pradhyumna Kumar Singh,

Prabodh Kumar Trivedi,

Pravendra Nath,

Rakesh Tuli

Publication year - 2010

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.109.150979

Subject(s) - phenylpropanoid , biology , arabidopsis , spodoptera litura , nicotiana tabacum , transcriptome , arabidopsis thaliana , transcription factor , genetically modified crops , plant defense against herbivory , metabolome , transgene , secondary metabolism , gene , metabolic pathway , genetics , botany , gene expression , metabolomics , biosynthesis , bioinformatics , larva , mutant

Flavonoids synthesized by the phenylpropanoid pathway participate in myriad physiological and biochemical processes in plants. Due to the diversity of secondary transformations and the complexity of the regulation of branched pathways, single gene strategies have not been very successful in enhancing the accumulation of targeted molecules. We have expressed an Arabidopsis (Arabidopsis thaliana) transcription factor, AtMYB12, in tobacco (Nicotiana tabacum), which resulted in enhanced expression of genes involved in the phenylpropanoid pathway, leading to severalfold higher accumulation of flavonols. Global gene expression and limited metabolite profiling of leaves in the transgenic lines of tobacco revealed that AtMYB12 regulated a number of pathways, leading to flux availability for the phenylpropanoid pathway in general and flavonol biosynthesis in particular. The tobacco transgenic lines developed resistance against the insect pests Spodoptera litura and Helicoverpa armigera due to enhanced accumulation of rutin. Suppression of flavonol biosynthesis by artificial microRNA reversed insect resistance of the AtMYB12-expressing tobacco plants. Our study suggests that AtMYB12 can be strategically used for developing safer insect pest-resistant transgenic plants.

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