A Microscale Technique for Gas Chromatography-Mass Spectrometry Measurements of Picogram Amounts of Indole-3-Acetic Acid in Plant Tissues | Zendy

Agneta Edlund | Zendy; Staffan Eklöf | Zendy; Birgitta Sundberg | Zendy; Thomas Möritz | Zendy; Göran Sandberg | Zendy

Open Access

A Microscale Technique for Gas Chromatography-Mass Spectrometry Measurements of Picogram Amounts of Indole-3-Acetic Acid in Plant Tissues

Author(s) -

Agneta Edlund,

Staffan Eklöf,

Birgitta Sundberg,

Thomas Möritz,

Göran Sandberg

Publication year - 1995

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.108.3.1043

Subject(s) - nicotiana tabacum , microscale chemistry , chemistry , indole 3 acetic acid , mass spectrometry , chromatography , gas chromatography , acetic acid , selectivity , gas chromatography–mass spectrometry , biochemistry , auxin , mathematics education , mathematics , gene , catalysis

A microscale technique has been developed for routine quantifications of picogram amounts of indole-3-acetic acid (IAA) in plant tissues by combined gas chromatography-mass spectrometry. Low- and high-resolution selected-ion-monitoring and selected-reaction-monitoring mass spectrometry techniques were compared for selectivity and precision. The best selectivity was obtained with selected-reaction-monitoring analysis, and 1-mg samples containing 500 fg of IAA could be analyzed accurately with this method. This technique was used to investigate the IAA distribution pattern along the longitudinal axis of tobacco (Nicotiana tabacum [L.]) leaves. In young, developing leaves an increase of endogenous IAA from the leaf tip to the base of the leaf was observed, whereas the level of IAA was uniform along this axis in mature leaves.

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