Subcellular Compartmentation of the 4-Aminobutyrate Shunt in Protoplasts from Developing Soybean Cotyledons | Zendy

Kevin E. Breitkreuz | Zendy; Barry J. Shelp | Zendy

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Subcellular Compartmentation of the 4-Aminobutyrate Shunt in Protoplasts from Developing Soybean Cotyledons

Author(s) -

Kevin E. Breitkreuz,

Barry J. Shelp

Publication year - 1995

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.108.1.99

Subject(s) - percoll , protoplast , biochemistry , differential centrifugation , biology , glutamate dehydrogenase , cytosol , glycine , subcellular localization , organelle , enzyme , photorespiration , vacuole , mitochondrion , centrifugation , amino acid , glutamate receptor , cytoplasm , receptor

The subcellular localization of enzymes involved in the 4-ami-nobutyrate shunt was investigated in protoplasts prepared from developing soybean [Glycine max (L.) Merrill cv Maple Arrow] cotyledons. Protoplast lysate was fractionated by differential and continuous Percoll-gradient centrifugation to separate organelle fractions. Glutamate decarboxylase (EC 4.1.1.15) was found exclusively in the cytosol, whereas 4-aminobutyrate:pyruvate transami-nase (EC 2.6.1.19) and succinic semialdehyde dehydrogenase (EC 1.2.1.16) were associated exclusively with the mitochondrial fractions. Mitochondrial fractions also catabolized [U-14C]4-aminobu-tyrate to labeled succinate.

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