Open AccessEffects of Temperature on Infected Cell O2 Concentration and Adenylate Levels in Attached Soybean Nodules
Author(s) -
Mónika Kuzma,
А. Ф. Топунов,
David B. Layzell
Publication year - 1995
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.107.4.1209
Subject(s) - leghemoglobin , energy charge , adenylate kinase , nodule (geology) , respiration , biology , oxygen , glycine , metabolism , energy metabolism , chemistry , biochemistry , root nodule , endocrinology , botany , nitrogen , nitrogen fixation , enzyme , paleontology , organic chemistry , amino acid
To assess the role of O2 in the regulation of nodule metabolism following a decrease or an increase in temperature, the fractional oxygenation of leghemoglobin (FOL) was measured in soybean (Glycine max L. Merr.) nodules during rapid and gradual changes in temperature from 20[deg]C to either 15 or 25[deg]C. The affinity of leghemoglobin for O2 was also measured at each temperature and the values were used to calculate the infected cell O2 concentration (Oi). After nodules were transferred to 15[deg]C, FOL and Oi increased and adenylate energy charge (AEC = [ATP + 0.5ADP]/[ATP + ADP + AMP]) increased from 0.70 to 0.78. The temperature increase was associated with a decrease in FOL and Oi. We concluded that changes in nodule temperature alter the respiratory demand of the nodules for O2, resulting in a change in Oi and a shift in the balance between ATP consumption and ATP production within the nodule tissue.