1 L-myo-Inositol 1-Phosphate Synthase from Arabidopsis thaliana | Zendy

Margaret Dean Johnson | Zendy; I. M. Sussex | Zendy

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1 L-myo-Inositol 1-Phosphate Synthase from Arabidopsis thaliana

Author(s) -

Margaret Dean Johnson,

I. M. Sussex

Publication year - 1995

Publication title -

plant physiology

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.107.2.613

Subject(s) - biochemistry , biology , saccharomyces cerevisiae , complementary dna , arabidopsis thaliana , yeast , arabidopsis , atp synthase , gene , inositol , microbiology and biotechnology , inositol phosphate , mutant , receptor

A recombinant phage containing an Arabidopsis thaliana cDNA sequence encoding a protein with 1L-myo-inositol 1-phosphate synthase (EC 5.5.1.4) activity has been isolated and used for transcriptional and translational studies. The identification of the recombinant phage relied on the observations that (a) the clone complements a mutation in the structural gene for 1L-myo-inositol 1-phosphate synthase in the yeast Saccharomyces cerevisiae, (b) the in vitro synthesized polypeptide enzymatically converts glucose 6-phosphate into inositol 1-phosphate, (c) in vitro transcription and translation of this cDNA sequence produces a polypeptide that is recognized by anti-yeast myo-inositol 1-phosphate synthase antiserum, and (d) inositol regulates the expression of the corresponding gene in Arabidopsis.

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