Identification of a Protein That Inhibits the Phosphorylated Form of Nitrate Reductase from Spinach (Spinacia oleracea) Leaves | Zendy

Carol MacKintosh | Zendy; Pauline Douglas | Zendy; Cathrine Lillo | Zendy

Open Access

Identification of a Protein That Inhibits the Phosphorylated Form of Nitrate Reductase from Spinach (Spinacia oleracea) Leaves

Author(s) -

Carol MacKintosh,

Pauline Douglas,

Cathrine Lillo

Publication year - 1995

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.107.2.451

Subject(s) - spinacia , dephosphorylation , spinach , nip , phosphorylation , biochemistry , nitrate reductase , phosphatase , protein phosphorylation , chemistry , protein kinase a , enzyme , biology , chloroplast , computer science , gene , programming language

The low-activity, phosphorylated form of nitrate reductase (NR) became activated during purification from spinach (Spinacia oleracea) leaves harvested in the dark. This activation resulted from its separation from an approximately 110-kd nitrate reductase inhibitor protein (NIP). Readdition of NIP inactivated the purified phosphorylated NR, but not the active dephosphorylated form of NR, indicating that the inactivation of NR requires its interaction with NIP as well as phosphorylation. Consistent with this hypothesis, NR that had been inactivated in vitro in the presence of NR kinase, ATP-Mg, and NIP could be reactivated either by dephosphorylation with protein phosphatase 2A or by dissociation of NIP from NR.

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