Global Characterization of Cell-Specific Gene Expression through Fluorescence-Activated Sorting of Nuclei  | Zendy

Changqing Zhang | Zendy; Roger Barthelson | Zendy; Georgina M. Lambert | Zendy; David W. Galbraith | Zendy

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Global Characterization of Cell-Specific Gene Expression through Fluorescence-Activated Sorting of Nuclei

Author(s) -

Changqing Zhang,

Roger Barthelson,

Georgina M. Lambert,

David W. Galbraith

Publication year - 2008

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.107.115246

Subject(s) - cell sorting , biology , gene , gene expression , fluorescence , cell , microbiology and biotechnology , sorting , identification (biology) , subcellular localization , transgene , computational biology , genetics , botany , physics , quantum mechanics , computer science , programming language

We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.

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