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Heterologous Expression of Viral RNA Interference Suppressors: RISC Management
Author(s) -
KarenBeth G. Scholthof
Publication year - 2007
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.107.106807
Subject(s) - suppressor , rna interference , biology , gene silencing , rna , heterologous , scope (computer science) , vector (molecular biology) , viral vector , computational biology , rna silencing , gene , plant virus , genetics , heterologous expression , computer science , virus , programming language , recombinant dna
This Update reviews how virus-encoded suppres- sors of RNA silencing are currently being used in cer- tain areas of plant research and biotechnology. Keeping within the scope of this special Focus issue, an empha- sis is placed on the use of gene vectors for expression of suppressors in plants. Toward the end of this re- view, a discussion is presented on how the choice of vector system may influence the functionality of sup- pressors and the occurrence of side effects. The pur- pose of this brief review is to illustrate, for a general audience of biologists, how individual properties of viral-encoded suppressors might affect their perfor- mance in plants when expressed by heterologous vectors.

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