Isolation and Characterization of cDNAs Encoding Imidazoleglycerolphosphate Dehydratase from Arabidopsis thaliana | Zendy

S. Tada | Zendy; S. Volrath | Zendy; D. Guyer | Zendy; Alfred Scheidegger | Zendy; John Ryals | Zendy; Daisaku Ohta | Zendy; E. R. Ward | Zendy

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Isolation and Characterization of cDNAs Encoding Imidazoleglycerolphosphate Dehydratase from Arabidopsis thaliana

Author(s) -

S. Tada,

S. Volrath,

D. Guyer,

Alfred Scheidegger,

John Ryals,

Daisaku Ohta,

E. R. Ward

Publication year - 1994

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.105.2.579

Subject(s) - dehydratase , arabidopsis thaliana , biology , complementation , complementary dna , arabidopsis , southern blot , molecular cloning , gene , microbiology and biotechnology , escherichia coli , biochemistry , mutant

cDNA clones encoding imidazoleglycerolphosphate dehydratase (IGPD; EC 4.2.1.19) from Arabidopsis thaliana were isolated by complementation of a bacterial auxotroph. The predicted primary translation product shared significant identity with the corresponding sequences from bacteria and fungi. As in yeast, the plant enzyme is monofunctional, lacking the histidinol phosphatase activity present in the Escherichia coli protein. IGPD mRNA was present in major organs at all developmental stages assayed. The Arabidopsis genome appears to contain two genes encoding this enzyme, based on DNA gel blot and polymerase chain reaction analysis.

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