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Loss of Microtubules in the Interphase Cells of Onion (Allium cepa L.) Root Tips from the Cell Cortex and Their Appearance in the Cytoplasm after Treatment with Cycloheximide
Author(s) -
Yoshinobu Mineyuki,
Hidetoshi Iida,
Yasutaka Anraku
Publication year - 1994
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.104.1.281
Subject(s) - cycloheximide , interphase , cytoplasm , microtubule , biology , allium , cortex (anatomy) , mitosis , microbiology and biotechnology , cell cortex , cell , botany , biophysics , biochemistry , protein biosynthesis , cytoskeleton , neuroscience
As part of a project to investigate the mechanism of cortical microtubule (MT) alignment, we examined the effects of cycloheximide (CHM) on cortical MTs in the root tip cells of Allium cepa L. Results show that although a preprophase band of MTs remained in the cell cortex, interphase MTs disappeared from the cortical cytoplasm and then appeared concomitantly in the inner cytoplasm when the rate of de novo protein synthesis was reduced with CHM (11-360 [mu]M for 2 h)

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