Open AccessRecognition of Peroxisomes by Immunofluorescence in Transformed and Untransformed Tobacco Cells
Author(s) -
Joanne Marrison,
I. Onyeocha,
Alison Baker,
Rachel M. Leech
Publication year - 1993
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.103.4.1055
Subject(s) - nicotiana tabacum , peroxisome , transgene , immunofluorescence , biology , enzyme , genetically modified crops , biochemistry , microbiology and biotechnology , antibody , gene , genetics
We report the visualization of peroxisomes in tobacco (Nicotiana tabacum) leaves using fluorescently labeled antibodies to glycolate oxidase. In transgenic tobacco leaves the expression of isocitrate lyase was also visualized. In dual probing experiments both enzymes were shown to be present together in all peroxisomes in transgenic tobacco leaves.