Open AccessExpression and Maintenance of Embryogenic Potential Is Enhanced through Constitutive Expression ofAGAMOUS-Like 15
Author(s) -
Ellen W. Harding,
Weining Tang,
Karl W. Nichols,
Donna E. Fernandez,
Sharyn E. Perry
Publication year - 2003
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.103.023499
Subject(s) - suspensor , biology , agamous , embryo , microbiology and biotechnology , somatic embryogenesis , microspore , meristem , zygote , ectopic expression , endosperm , embryogenesis , botany , transgene , ovule , gene expression , genetics , gene , shoot , stamen , homeotic gene , pollen
The MADS domain protein AGL15 (AGAMOUS-Like 15) has been found to preferentially accumulate in angiosperm tissues derived from double fertilization (i.e. the embryo, suspensor, and endosperm) and in apomictic, somatic, and microspore embryos. Localization to the nuclei supports a role in gene regulation during this phase of the life cycle. To test whether AGL15 is involved in the promotion and maintenance of embryo identity, the embryogenic potential of transgenic plants that constitutively express AGL15 was assessed. Expression of AGL15 was found to enhance production of secondary embryos from cultured zygotic embryos, and constitutive expression led to long-term maintenance of development in this mode. Ectopic accumulation of AGL15 also promoted somatic embryo formation after germination from the shoot apical meristem of seedlings in culture. These results indicate that AGL15 is involved in support of development in an embryonic mode.
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