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Identification of ArabidopsisratMutants
Author(s) -
Yanmin Zhu,
Jaesung Nam,
Jaime M. Humara,
Kirankumar S. Mysore,
Lan-Ying Lee,
Hongbin Cao,
Lisa Valentine,
Jingling Li,
Anthony D. Kaiser,
Andrea L. Kopecky,
HauHsuan Hwang,
Saikat Bhattacharjee,
Praveen Rao,
Tzvi Tzfira,
Jyothi Rajagopal,
HoChul Yi,
Veena,
Badam Singh Yadav,
Charles F. Crane,
Kui Lin,
Yves Larcher,
Matthew J.K. Gelvin,
Marnie Knue,
Cynthia Ramos,
Xiaowen Zhao,
S. J. Davis,
SangIc Kim,
C. T. Ranjith-Kumar,
Yoo Jin Choi,
Vipin Hallan,
Sudip Chattopadhyay,
Xiangzhen Sui,
Alicja Ziemienowicz,
Ann G. Matthysse,
Vitaly Citovsky,
Barbara Höhn,
Stanton B. Gelvin
Publication year - 2003
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.103.020420
Subject(s) - arabidopsis , mutant , biology , transformation (genetics) , gene , genetics , agrobacterium , chromatin , microbiology and biotechnology , computational biology
Limited knowledge currently exists regarding the roles of plant genes and proteins in the Agrobacterium tumefaciens-mediated transformation process. To understand the host contribution to transformation, we carried out root-based transformation assays to identify Arabidopsis mutants that are resistant to Agrobacterium transformation (rat mutants). To date, we have identified 126 rat mutants by screening libraries of T-DNA insertion mutants and by using various "reverse genetic" approaches. These mutants disrupt expression of genes of numerous categories, including chromatin structural and remodeling genes, and genes encoding proteins implicated in nuclear targeting, cell wall structure and metabolism, cytoskeleton structure and function, and signal transduction. Here, we present an update on the identification and characterization of these rat mutants.

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