Endopolygalacturonase in Apples (Malus domestica) and Its Expression during Fruit Ripening | Zendy

Qiuyan Wu | Zendy; M. SzakäcsDobozi | Zendy; M. Hemmat | Zendy; G. Hrazdina | Zendy

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Endopolygalacturonase in Apples (Malus domestica) and Its Expression during Fruit Ripening

Author(s) -

Qiuyan Wu,

M. SzakäcsDobozi,

M. Hemmat,

G. Hrazdina

Publication year - 1993

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.102.1.219

Subject(s) - ripening , pectinase , malus , complementary dna , enzyme assay , gel electrophoresis , polyacrylamide gel electrophoresis , enzyme , chemistry , biochemistry , pectin , biology , size exclusion chromatography , microbiology and biotechnology , horticulture , gene

The activity of polygalacturonase (PG) has been detected in ripe McIntosh apples (Malus domestica Borkh. cv McIntosh) both by enzyme activity measurement and immunoblotting using an anti-tomato-PG antibody preparation. PG activity increased during fruit ripening and remained steady, or decreased slightly, after 5 months of controlled atmospheric storage. The enzyme had a relative molecular weight of 45,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 56,000 to 61,000 when determined by gel filtration. Viscosity and reducing end group measurements with a commercial pectin preparation showed that the enzyme is endo acting. In RNA and DNA blot hybridization experiments, a full-length tomato PG cDNA hybridized with the apple RNA and DNA, showing the identity of genes encoding the activity of the enzyme in tomato and apple.

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