Electrically Induced Protoplast Fusion Using Pulse Electric Fields for Dielectrophoresis | Zendy

Antoaneta P. Dimitrova | Zendy; Alexander Christov | Zendy

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Electrically Induced Protoplast Fusion Using Pulse Electric Fields for Dielectrophoresis

Author(s) -

Antoaneta P. Dimitrova,

Alexander Christov

Publication year - 1992

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.100.4.2008

Subject(s) - protoplast , electrofusion , dielectrophoresis , fusion , pulse (music) , electric field , pisum , nuclear fusion , cell fusion , biophysics , analytical chemistry (journal) , physics , chemistry , biology , botany , materials science , atomic physics , chromatography , biochemistry , optics , cell , composite material , linguistics , philosophy , quantum mechanics , detector

The formation of protoplast chains in suspensions of isolated pea (Pisum sativum cv Ran 1) mesophyll protoplasts induced by electric fields was studied. The chain formation induced by a sine-wave field (2 V, peak to peak; 500-0.1 kHz) was compared to that induced by an alternating pulse field (1 V, amplitude; 0.1-0.4 kHz). An increased number of dielectrophoretically paired protoplasts, formation of protoplast chains in the presence of CaCl(2) up to 5 mm, and protoplast fusion in the presence of 3 mm CaCl(2) were found when the pulse field was applied. The present results suggest the possibility of electrically induced protoplast fusion at cation concentrations that prevent fusion when sine-wave fields are applied.

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