Open AccessEstimation of Ammonium Ion Distribution between Cytoplasm and Vacuole Using Nuclear Magnetic Resonance Spectroscopy
Author(s) -
Justin K. M. Roberts,
Maynard K. L. Pang
Publication year - 1992
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.100.3.1571
Subject(s) - vacuole , cytoplasm , ammonium , extracellular , intracellular , chemistry , biophysics , biochemistry , biology , organic chemistry
Evidence is presented that intracellular ammonium is trapped in vacuoles of maize (Zea mays L.) root tips because of rapid movement of ammonia between cytoplasm and vacuoles. The concentration of cytoplasmic ammonium is estimated to be <15 mum at extracellular ammonium concentrations up to 1 mm. The implications for pathways of ammonium assimilation are discussed.
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