Open AccessInduction of a Putative Ca2+-ATPase mRNA in NaCl-Adapted Cells
Author(s) -
Eva Perez-Prat,
Meena L. Narasimhan,
Marla L. Binzel,
Miguel A. Botella,
Zutang Chen,
Victoriano Valpuesta,
Ray A. Bressan,
Paul M. Hasegawa
Publication year - 1992
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.100.3.1471
Subject(s) - messenger rna , atpase , chemistry , biophysics , microbiology and biotechnology , biochemistry , enzyme , biology , gene
A cDNA clone was isolated that encodes the partial sequence of a putative endoplasmic reticulum Ca(2+)-ATPase of tobacco. The 1.497-kb insert had an open reading frame of 1.149 kb. The deduced peptide had the greatest homology to the endoplasmic reticulum Ca(2+)-ATPases of Drosophila and Artemia, followed by the mammalian and avian enzymes (SERCA2 and 3). The cDNA insert hybridized to a single mRNA of 4.4 kb from tobacco cultured cells or plant tissues. The level of this transcript was induced about 2-fold by NaCl shock in 428 mm NaCl-deadapted tobacco cells that were maintained in medium without salt, but not in unadapted cells. The level of this transcript was 3- to 4-fold higher in 428 mm NaCl-adapted cells growing in salt than in unadapted cells growing without salt.