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Pressure Clamp Method to Measure Transpiration in Growing Single Plant Cells
Author(s) -
Joseph K. E. Ortega,
Scott Bell,
Ada J. Erazo
Publication year - 1992
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.100.2.1036
Subject(s) - transpiration , clamp , botany , biology , photosynthesis , mechanical engineering , clamping , engineering
A pressure probe method (pressure clamp) was developed to measure transpiration rates of both growing and nongrowing single plant cells, and represents an improvement over the previous pressure probe method (pressure relaxation), which is restricted to nongrowing plant cells (J.K.E. Ortega, R.G. Keanini, K.J. Manica [1988] Plant Physiol 87: 11-14). The pressure clamp method was used to measure transpiration rates of Phycomyces sporangiophores in two developmental stages: stage III (nongrowing) and stage IV (growing).

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