A New Method of Measuring Protein-Methionine-S-Oxide Reductase Activity | Zendy

David Ferguson | Zendy; John Burke | Zendy

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A New Method of Measuring Protein-Methionine-S-Oxide Reductase Activity

Author(s) -

David Ferguson,

John Burke

Publication year - 1992

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.100.1.529

Subject(s) - methionine , pisum , reductase , enzyme , chromatography , biochemistry , chemistry , substrate (aquarium) , enzyme assay , biology , amino acid , ecology

A new assay for measuring protein-methionine-S-oxide reductase is reported. The assay measures the conversion of N-(9-fluorenylmethoxycarbonyl)-methionine sulfoxide to N-(9-fluorenylmethoxycarbonyl)-methionine using fluorescence detection after high-performance liquid chromatography separation. Enzyme activity is linear over a 60-min period and the assay is sensitive enough to detect the consumption of only 1% of available substrate. Protein-methionine-S-oxide reductase activity was demonstrated in pea (Pisum sativum L.) chloroplasts, and enzyme levels in leaves of etiolated and light-grown seedlings were compared.

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