Inactivation of the Phloem-Specific Dof Zinc Finger GeneDAG1Affects Response to Light and Integrity of the Testa of Arabidopsis Seeds | Zendy

Maura Papi | Zendy; Sabrina Sabatini | Zendy; Maria Maddalena Altamura | Zendy; Lars Hennig | Zendy; Eberhard Schäfer | Zendy; Paolo Costantino | Zendy; Paola Vittorioso | Zendy

Open Access

Inactivation of the Phloem-Specific Dof Zinc Finger GeneDAG1Affects Response to Light and Integrity of the Testa of Arabidopsis Seeds

Author(s) -

Maura Papi,

Sabrina Sabatini,

Maria Maddalena Altamura,

Lars Hennig,

Eberhard Schäfer,

Paolo Costantino,

Paola Vittorioso

Publication year - 2002

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.010488

Subject(s) - arabidopsis , phytochrome , mutant , zinc finger , phloem , biology , germination , gene , microbiology and biotechnology , zinc finger transcription factor , arabidopsis thaliana , botany , transcription factor , genetics , red light

We show here that seeds from the knockout mutant of the Arabidopsis DAG1 gene encoding a Dof zinc finger transcription factor have an altered response to red and far-red light. Mutant dag1 seeds are induced to germinate by much lower red light fluence rates, and germination reaches more quickly a point where it is independent of phytochrome signaling. Moreover, although microscopic analysis reveals no obvious structural alterations in the seed coat (testa) of dag1 seeds, staining assays with different dyes point to an abnormal fragility of the testa. By extensive in situ mRNA hybridization analysis we show here that the gene, which is not expressed in the embryo, is specifically expressed in the phloem of all organs of the mother plant.

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