Open AccessSoft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell
Author(s) -
Xiaojing Huang,
Johanelson Weker,
Janos Kirz,
Enju Lima,
Stefano Marchesini,
Huijie Miao,
Aaron M. Neiman,
David A. Shapiro,
Jan Steinbrener,
Andrew Stewart,
Joshua J. Turner,
Chris Jacobsen
Publication year - 2009
Publication title -
physical review letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.688
H-Index - 673
eISSN - 1079-7114
pISSN - 0031-9007
DOI - 10.1103/physrevlett.103.198101
Subject(s) - diffraction , resolution (logic) , microscopy , x ray crystallography , materials science , crystallization , optics , coherent diffraction imaging , x ray , yeast , budding yeast , crystallography , chemistry , physics , saccharomyces cerevisiae , phase retrieval , biochemistry , organic chemistry , fourier transform , quantum mechanics , computer science , artificial intelligence
We report the first image of an intact, frozen hydrated eukaryotic cell using x-ray diffraction microscopy, or coherent x-ray diffraction imaging. By plunge freezing the specimen in liquid ethane and maintaining it below -170 degrees C, artifacts due to dehydration, ice crystallization, and radiation damage are greatly reduced. In this example, coherent diffraction data using 520 eV x rays were recorded and reconstructed to reveal a budding yeast cell at a resolution better than 25 nm. This demonstration represents an important step towards high resolution imaging of cells in their natural, hydrated state, without limitations imposed by x-ray optics.
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