Maintaining Live Cells and Tissue Slices in the Imaging Setup
Author(s) -
Michael E. Dailey,
Glen S. Marrs,
Dana Kurpius
Publication year - 2011
Publication title -
cold spring harbor protocols
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 51
eISSN - 1940-3402
pISSN - 1559-6095
DOI - 10.1101/pdb.top105
Subject(s) - live cell imaging , ex vivo , computer science , simple (philosophy) , biomedical engineering , biology , medicine , in vivo , cell , philosophy , genetics , microbiology and biotechnology , epistemology
Live imaging provides exciting opportunities to study dynamic cellular events as they occur in real time. However, such experimental procedures present several challenges. This chapter discusses some of the major considerations relating to the maintenance of live biological samples during ex vivo imaging and presents some relatively simple, low-cost solutions to keeping samples healthy.
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