Open AccessLigation and Ligases
Author(s) -
Michael R. Green,
Joseph Sambrook
Publication year - 2019
Publication title -
cold spring harbor protocols
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 51
eISSN - 1940-3402
pISSN - 1559-6095
DOI - 10.1101/pdb.top101352
Subject(s) - ligation , nucleic acid , bacteriophage , cloning (programming) , dna , molecular cloning , computational biology , chemistry , biology , microbiology and biotechnology , biochemistry , computer science , escherichia coli , gene , peptide sequence , programming language
DNA ligases are used chiefly to create novel combinations of nucleic acid molecules and to attach them to vectors before molecular cloning. They are either of bacterial origin or bacteriophage encoded and have different properties, as discussed here.
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