Open AccessDigoxigenin
Author(s) -
Michael R. Green,
Joseph Sambrook
Publication year - 2021
Publication title -
cold spring harbor protocols
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 51
eISSN - 1940-3402
pISSN - 1559-6095
DOI - 10.1101/pdb.top100784
Subject(s) - digoxigenin , nucleic acid , microbiology and biotechnology , chemistry , rna , conjugate , biology , biochemistry , in situ hybridization , messenger rna , mathematics , gene , mathematical analysis
In molecular cloning, digoxigenin is used as a ligand that can be incorporated into DNA and RNA probes and detected after hybridization with an anti-digoxigenin-antibody enzyme conjugate. Methods to label nucleic acids with digoxigenin and to detect digoxigenin-labeled probes are introduced here.
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