Transcriptomics and Proteomics Methods for Xenopus Embryos and Tissues | Zendy

Michael J. Gilchrist | Zendy; Gert Jan C. Veenstra | Zendy; Ken W.Y. Cho | Zendy

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Transcriptomics and Proteomics Methods for Xenopus Embryos and Tissues

Author(s) -

Michael J. Gilchrist,

Gert Jan C. Veenstra,

Ken W.Y. Cho

Publication year - 2019

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.top098350

Subject(s) - proteomics , xenopus , computational biology , quantitative proteomics , transcriptome , biology , rna , protein expression , embryo , rna seq , gene expression , microbiology and biotechnology , gene , bioinformatics , genetics

The general field of quantitative biology has advanced significantly on the back of recent improvements in both sequencing technology and proteomics methods. The development of high-throughput, short-read sequencing has revolutionized RNA-based expression studies, while improvements in proteomics methods have enabled quantitative studies to attain better resolution. Here we introduce methods to undertake global analyses of gene expression through RNA and protein quantification in Xenopus embryos and tissues.

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